SingleRepair & Recovery

Thymosin Beta-4 10mg

Endogenous 43-amino-acid peptide evaluated in preclinical models for actin regulation, dermal healing, and cardioprotective signaling.

Mechanism

Binds and sequesters monomeric G-actin to govern cytoskeletal dynamics, cell migration, downstream angiogenesis, and ILK/Akt-mediated survival pathways.

Dosing

Standard laboratory protocols initiate research with a conservative loading dose of 2 mg administered subcutaneously twice per week.

Reconstitution

Reconstituting a 10 mg vial with 2.0 mL of bacteriostatic water yields a finished working concentration of 5 mg/mL.

Storage

Store the unmixed lyophilized powder chilled or frozen shielded from illumination, and maintain the reconstituted liquid at 2 to 8 °C for up to 28 days.

Mix & measure Thymosin Beta-4 10mg

Pre-filled with this protocol's recommended BAC water and documented starting dose — edit any field to run your own numbers.

Mix & measure Thymosin Beta-4 10mg

Vial strength → BAC water → target dose

Vial strength

BAC water

Target dose

Concentration5.00mg/mL

Draw volume0.400mL

On the syringe40.0units

Total doses in vial: 5.0

100u80u60u40u20u0

U-100 syringe: 100 units = 1 mL

Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Advanced calculator →

Dosing Chart

Phase / Day(s)	Dose & Frequency	Volume (U-100 units / mL)
Loading (conservative)	2 mg twice weekly	40 units (0.40 mL)
Loading (upper reference)	2.5 mg twice weekly	50 units (0.50 mL)
Maintenance	2 mg every 1–2 weeks	40 units (0.40 mL)

Reconstitution Steps

1
Thoroughly disinfect the rubber stoppers of both the Thymosin Beta-4 vial and bacteriostatic water vial using sterile alcohol wipes.
2
Draw 2.0 mL of bacteriostatic water into a sterile mixing syringe.
3
Insert the needle at a slight angle and introduce the diluent gently against the inner glass wall to prevent agitation of the lyophilized cake.
4
Carefully swirl the vial in circular motions until the lyophilized peptide is fully dissolved into a clear solution without shaking.
5
Affix a date label and place the reconstituted vial into refrigerated storage between 2 and 8 °C.

Supplies Needed

Peptide Vial

Contains 10 mg of lyophilized Thymosin Beta-4 research peptide.

Insulin Syringes (U-100)

Used for precise measurement and subcutaneous administration of research doses.

Bacteriostatic Water

Serves as the sterile diluent containing 0.9% benzyl alcohol for peptide reconstitution.

Alcohol Swabs

Ensures antiseptic preparation of vial stoppers and injection sites.

Why researchers study it

Dermal wound healing and collagen deposition

Cellular motility and angiogenic signaling

Cardiomyocyte survival and cardioprotection

Differentiation between full-length Tβ4 and synthetic actin-binding fragments

These describe what is being studied, not proven benefits, approved uses, or promised results.

Overview

Thymosin Beta-4 (Tβ4) is a ubiquitous, naturally occurring 43-amino-acid peptide that serves as the predominant monomeric G-actin sequestering molecule across mammalian systems. By buffering intracellular actin availability, it plays a fundamental role in directing cellular dynamics, structural reorganization, and motility critical for tissue remodeling. Unlike synthetic fragments commonly designated as TB-500, Tβ4 represents the intact parent peptide carrying multifaceted physiological actions throughout bodily systems. In investigative laboratory settings, external application of Tβ4 displays potent paracrine behaviors, stimulating angiogenesis, attenuating inflammatory cascades, and reducing fibrotic tissue generation. Much of the primary literature focuses on dermal repair assays in diabetic or aged models, alongside myocardial protection via integrin-linked kinase signaling pathways following ischemic trauma. Although subject to initial phase 1 and 2 human explorations for chronic ulcer healing and ophthalmic conditions, it remains exclusively an unapproved research compound without regulatory sanction for general clinical recovery.

References

1.Mannherz & Hannappel, Cell Motil Cytoskeleton, 2009 — The beta-thymosins: intracellular and extracellular activities of a versatile actin binding protein family
2.Bock-Marquette et al., Nature, 2004 — Thymosin beta4 activates integrin-linked kinase and promotes cardiac cell migration, survival and cardiac repair
3.Philp et al., Wound Repair Regen, 2003 — Thymosin beta 4 and a synthetic peptide containing its actin-binding domain promote dermal wound repair in db/db diabetic and aged mice
4.Kleinman & Sosne, Vitamins and Hormones, 2016 — Thymosin β4 Promotes Dermal Healing
5.Vasilopoulou et al., Expert Opin Biol Ther, 2015 — The role of thymosin-β4 in kidney disease