SingleImmune & Gut

Thymogen 10mg

Synthetic thymic dipeptide researched for its configuration-dependent immunomodulatory and bone marrow regenerative properties.

Mechanism

Thymogen operates as a stereospecific dipeptide that modulates cellular immunity and accelerates hematopoietic regeneration within bone marrow microenvironments.

Dosing

The documented reference starting dose in historical literature is approximately 100 mcg administered once daily over a short experimental window of 3 to 10 days.

Reconstitution

Reconstituting a 10 mg vial with 2 mL of bacteriostatic water results in a final concentration of 5 mg/mL.

Storage

Store the unmixed lyophilized powder refrigerated protected from light, and maintain the reconstituted liquid between 2 and 8 °C without freezing.

Mix & measure Thymogen 10mg

Pre-filled with this protocol's recommended BAC water and documented starting dose — edit any field to run your own numbers.

Mix & measure Thymogen 10mg

Vial strength → BAC water → target dose

Vial strength

BAC water

Target dose

Concentration5.00mg/mL

Draw volume0.020mL

On the syringe2.00units

Total doses in vial: 100.0

100u80u60u40u20u0

U-100 syringe: 100 units = 1 mL

Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Advanced calculator →

Dosing Chart

Phase / Day(s)	Dose & Frequency	Volume (U-100 units / mL)
Clinical-Equivalent Reference	100 mcg daily	0.02 mL (2 units)
Intermediate Research Level	250 mcg daily	0.05 mL (5 units)
High Experimental Benchmark	1 mg single point	0.20 mL (20 units)
Complete Vial Reference	10 mg total capacity	2.00 mL (200 units)

Reconstitution Steps

1
Clean the rubber stoppers of both the Thymogen vial and the bacteriostatic water vial using sterile alcohol prep pads and allow them to air-dry completely.
2
Draw 2 mL of bacteriostatic water using an appropriate reconstitution syringe.
3
Carefully introduce the needle into the peptide vial, allowing the diluent to run gently down the inside glass wall to avoid foaming the dipeptide cake.
4
Swirl the vial gently or roll it smoothly between your palms until the powder dissolves fully into a clear liquid; never shake the solution vigorously.
5
Inspect visually for any signs of residual particulate matter or discoloration, then place the solution into refrigerated storage at 2–8 °C.

Supplies Needed

Peptide Vial

Contains 10 mg of lyophilized Thymogen dipeptide for laboratory reconstitution.

Insulin Syringes (U-100)

Used to precisely draw low microgram-equivalent volumes (such as 0.02 mL / 2 units).

Bacteriostatic Water

Sterile diluent containing 0.9% benzyl alcohol required to reconstitute the lyophilized powder.

Alcohol Swabs

Used to sanitize vial stoppers and work surfaces prior to dilution and fluid transfers.

Why researchers study it

Thymic-mediated immunomodulation

Hematopoietic regeneration in irradiated marrow

Configuration-dependent peptide bioregulation

Microgram-range low-concentration biological activity

These describe what is being studied, not proven benefits, approved uses, or promised results.

Overview

Thymogen is a synthetic dipeptide composed of alpha-L-glutamyl-L-tryptophan (Glu-Trp). Originally synthesized as part of the Russian peptide bioregulator initiative, it was designed to emulate the immunoregulatory signaling pathways naturally regulated by the thymus gland. The molecule serves as an ultra-low-molecular-weight analog to complex organ extracts such as Thymalin, providing a streamlined tool for investigating cellular immune responses in laboratory models. Crucially, experimental protocols must differentiate Thymogen from golotimod (SCV-07), which features a gamma-D-Glu-L-Trp stereo-orientation. Pharmacological studies demonstrate that the specific optical isomerism governs its biological activity, meaning findings from D-isomer analogs cannot be translated to Thymogen research. While authorized within Russia for clinical use, it remains strictly an unapproved experimental bioregulator across Western regulatory domains. Investigational designs emphasize microgram-level interactions rather than standard milligram regimes. A standard 10 mg research vial contains numerous experimental single doses, requiring precise dilution protocols to ensure volumetric accuracy during testing in cell cultures or animal models.

References

1.Deigin et al., Immunology Letters, 1999 — Reciprocal effect of optical isomerism of EW-dipeptides on immune response
2.Okovityi & Gaivoronskaya, Eksperimental’naya i Klinicheskaya Farmakologiya, 2002 — Immune mechanisms of hepatoprotector effects of etomerzol and thymogen
3.Khavinson, Kuznik & Ryzhak, Advances in Gerontology, 2013 — Peptide bioregulators: a new class of geroprotectors (clinical studies results)
4.Khavinson et al., Molecules, 2020 — Peptides: prospects for use in the treatment of COVID-19