Humanin 5mg
Mitochondrial-derived peptide and potent HNG analog researched for cellular survival, metabolic signaling, and cytoprotective pathways.
Mechanism
Humanin mediates cytoprotection by interacting with cell-surface GP130/IL6ST receptor complexes to trigger downstream STAT3, AKT, and ERK1/2 survival pathways while safeguarding mitochondrial membrane potential.
Dosing
Preclinical community protocols document a conservative baseline of 500 mcg administered subcutaneously two times per week.
Reconstitution
Reconstituting a 5 mg vial with 2.0 mL of bacteriostatic water yields a finished working concentration of 2.5 mg/mL (2,500 mcg/mL).
Storage
Store unmixed lyophilized vials refrigerated or frozen protected from light, and maintain reconstituted solutions at 2–8°C for use within 28 days.
Mix & measure Humanin 5mg
Pre-filled with this protocol's recommended BAC water and documented starting dose — edit any field to run your own numbers.
Mix & measure Humanin 5mg
Vial strength → BAC water → target dose
Common vial sizes
Label claim
Total doses in vial: 10.0
U-100 syringe: 100 units = 1 mL
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Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Advanced calculator →
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Dosing Chart
| Phase / Day(s) | Dose & Frequency | Volume (U-100 units / mL) |
|---|---|---|
| Conservative start | 500 mcg, 2× weekly | 20 units (0.20 mL) |
| Common range | 1000 mcg, 2–3× weekly | 40 units (0.40 mL) |
| Cycle length | 4–8 weeks, then off | — |
Reconstitution Steps
- 1
Clean the rubber stoppers of both the Humanin peptide vial and the bacteriostatic water container with individual, sterile alcohol swabs and allow to dry.
- 2
Draw 2.0 mL of bacteriostatic water using a sterile syringe.
- 3
Insert the needle at a slight angle and discharge the diluent gently against the inner glass wall to prevent excessive foaming.
- 4
Swirl the vial with gentle circular motions until the lyophilized cake is completely dissolved; do not shake vigorously.
- 5
Inspect the clear solution, note the preparation date on the label, and place the vial into refrigerated storage at 2–8°C.
Supplies Needed
Peptide Vial
5 mg lyophilized Humanin (or HNG analog) source compound
Insulin Syringes (U-100)
Calibrated precision syringes for accurate microgram volume measurements
Bacteriostatic Water
Sterile diluent containing 0.9% benzyl alcohol for peptide solubilization
Alcohol Swabs
Antiseptic wipes for sterilizing vial stoppers and preparation surfaces
Why researchers study it
Neuroprotective actions against cellular amyloid-beta toxicity
Hypothalamic STAT3-mediated insulin sensitivity and glucose control
Correlation with longevity and healthspan markers across model organisms
Mitochondrial resilience in degenerative cellular models
These describe what is being studied, not proven benefits, approved uses, or promised results.
Overview
Humanin is an endogenous peptide encoded within the 16S ribosomal RNA region of mitochondrial DNA, consisting of 24 amino acids. Identified primarily as a cytoprotective factor, humanin belongs to the broader class of mitochondrial-derived peptides (MDPs), which act as functional retrograde signaling molecules coordinating cellular defense mechanisms, metabolic homeostasis, and survival under diverse physiological stressors. In laboratory environments, research frequently utilizes the synthetic variant HNG (S14G-Humanin), where a single glycine-for-serine substitution elevates bioactivity roughly a thousand-fold compared to native humanin. Experimental investigations center on its capacity to preserve mitochondrial integrity, attenuate programmed cell death, and influence central regulation of insulin sensitivity across preclinical rodent and cellular systems.
References
- 1.Hashimoto et al., J Neurosci, 2001 — Neuroprotection by the rescue factor humanin against Alzheimer's-relevant insults
- 2.Muzumdar et al., PLoS One, 2009 — Humanin: a novel central regulator of peripheral insulin action
- 3.Jin et al., Neurochem Int, 2009 — [Gly14]-Humanin protects against beta-amyloid via mitochondria
- 4.Kim et al., Oncotarget, 2016 — Humanin activates ERK1/2, AKT and STAT3 with age-dependent signaling
- 5.Nashine & Kenney, Cells, 2020 — Mitochondrial-Derived Peptides (MDPs) and cellular health in AMD
- 6.Yen et al., Aging (Albany NY), 2020 — Humanin is a regulator of lifespan and healthspan
Supplies Needed
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