SingleLongevity

N-Acetyl Epitalon Amidate 5mg

N-terminally acetylated and C-terminally amidated analog of Epitalon studied in cellular aging and epigenetic research models.

Mechanism

The peptide is proposed to modulate epigenetic transcription by preferentially interacting with histones H1/3 and H1/6, thereby influencing downstream telomerase catalytic subunit expression and cellular senescence pathways.

Dosing

Preclinical surface-area scaled models benchmark an initial research reference amount of 230 mcg administered via intermittent regimens.

Reconstitution

Reconstitution of a 5 mg vial with 2.0 mL of bacteriostatic water yields an active working concentration of 2.5 mg/mL (25 mcg per U-100 unit).

Storage

Store the unmixed lyophilized vial refrigerated or frozen away from light, and maintain the reconstituted liquid at 2–8°C without freezing.

Mix & measure N-Acetyl Epitalon Amidate 5mg

Pre-filled with this protocol's recommended BAC water and documented starting dose — edit any field to run your own numbers.

Mix & measure N-Acetyl Epitalon Amidate 5mg

Vial strength → BAC water → target dose

Vial strength

BAC water

Target dose

Concentration2.50mg/mL

Draw volume0.092mL

On the syringe9.20units

Total doses in vial: 21.7

100u80u60u40u20u0

U-100 syringe: 100 units = 1 mL

Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Advanced calculator →

Dosing Chart

Phase / Day(s)	Dose & Frequency	Volume (U-100 units / mL)
Preclinical Equivalent	230 mcg intermittent cycle	9 units (0.09 mL)
Intermediate Reference	500 mcg intermittent cycle	20 units (0.20 mL)
Milligram Benchmark	1 mg intermittent cycle	40 units (0.40 mL)
Full Vial Baseline	5 mg single evaluation	200 units (2.00 mL)

Reconstitution Steps

1
Allow the 5 mg lyophilized peptide vial and the bacteriostatic water to equilibrate to ambient room temperature.
2
Disinfect the rubber stoppers of both vials using separate sterile 70% isopropyl alcohol swabs.
3
Draw exactly 2.0 mL of bacteriostatic water using a sterile reconstitution syringe.
4
Insert the needle into the peptide vial at an angle, directing the fluid slowly down the glass wall rather than directly onto the powder cake.
5
Gently rotate the vial in a circular motion until the lyophilisate dissolves completely into a clear liquid; avoid vigorous shaking to prevent shear degradation.
6
Record the reconstitution date and final concentration (2.5 mg/mL) on the vial label and place immediately into refrigerated storage at 2–8°C.

Supplies Needed

Peptide Vial

Lyophilized N-Acetyl Epitalon Amidate 5 mg container for laboratory research.

Insulin Syringes (U-100)

Precision measuring tool for accurate micro-volume unit extraction.

Bacteriostatic Water

Preserved sterile diluent containing 0.9% benzyl alcohol for multi-dose reconstitution.

Alcohol Swabs

Antiseptic pads used to decontaminate rubber vial stoppers before needle entry.

Why researchers study it

Telomerase activation and telomere length dynamics in somatic cell models

Epigenetic modulation and histone interaction pathways

Terminal peptide capping strategies for enzymatic stability

Preclinical longevity and age-associated cellular senescence markers

These describe what is being studied, not proven benefits, approved uses, or promised results.

Overview

N-Acetyl Epitalon Amidate is a synthetically modified tetrapeptide derived from Epitalon (Ala-Glu-Asp-Gly). By incorporating an acetyl group at the N-terminus and an amide group at the C-terminus, the molecule is theoretically engineered to resist enzymatic breakdown by exopeptidases, such as aminopeptidases and carboxypeptidases. Despite these synthetic adaptations, the analog lacks direct pharmacokinetic or comparative peer-reviewed research confirming enhanced stability over its parent compound. Investigations into this peptide class generally extrapolate from preclinical studies on unmodified Epitalon. In non-human models and in vitro cell cultures, the sequence has been examined for its effects on telomerase expression, cellular senescence markers, and chromatin remodeling. However, independent replication and formal clinical evaluations remain absent in registered regulatory databases. Laboratory researchers investigating this modified variant frequently navigate broad discrepancies between in vivo animal doses and empirical market conventions. Standardized laboratory documentation emphasizes precise reconstitution math, sterile handling techniques, and controlled environmental storage to maintain the structural integrity of the lyophilisate.

References

1.Anisimov et al., Biogerontology, 2003 — Biomarkers of aging, life span, and spontaneous tumor incidence in female SHR mice treated with Epitalon
2.Khavinson, Bondarev & Butyugov, Bulletin of Experimental Biology and Medicine, 2003 — Epithalon induction of telomerase activity and telomere elongation in human somatic cells
3.Khavinson et al., Bulletin of Experimental Biology and Medicine, 2004 — AEDG peptide promotion of extended division limits in fetal somatic fibroblasts
4.Khavinson et al., Molecules, 2020 — Epigenetic mechanisms and histone-binding dynamics of Epitalon in mesenchymal stem cells
5.Semenchenko, Anisimov & Yashin, Experimental Gerontology, 2004 — Mathematical modeling of longevity hormesis in HER-2/neu transgenic mice