SingleLongevity

Glutathione 1500mg

Endogenous tripeptide antioxidant researched for cellular redox defense, hepatic conjugation pathways, and pigment regulation.

Mechanism

Functions as a fundamental intracellular thiol antioxidant that neutralizes reactive oxygen species and acts as an essential substrate in enzymatic detoxification and conjugation pathways.

Dosing

Laboratory protocols document an initial dose of 100 mg administered two to three times weekly.

Reconstitution

Reconstituting a 1500 mg vial with 7.5 mL of bacteriostatic water yields a working concentration of 200 mg/mL.

Storage

Store unmixed lyophilized vials at -20°C in a dry environment protected from light, and keep the reconstituted solution refrigerated at 2–8°C without freezing.

Mix & measure Glutathione 1500mg

Pre-filled with this protocol's recommended BAC water and documented starting dose — edit any field to run your own numbers.

Mix & measure Glutathione 1500mg

Vial strength → BAC water → target dose

Vial strength

BAC water

Target dose

Concentration200.0mg/mL

Draw volume0.500mL

On the syringe50.0units

Total doses in vial: 15.0

100u80u60u40u20u0

U-100 syringe: 100 units = 1 mL

Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Advanced calculator →

Dosing Chart

Phase / Day(s)	Dose & Frequency	Volume (U-100 units / mL)
Weeks 1–2 (initiation)	100 mg (2–3× weekly)	50 units (0.50 mL)
Weeks 3–8 (maintenance)	200 mg (2–3× weekly)	100 units (1.0 mL)
Weeks 9–12	200 mg (2–3× weekly)	100 units (1.0 mL)

Reconstitution Steps

1
Aspirate 7.5 mL of bacteriostatic water into a sterile syringe.
2
Introduce the diluent slowly against the inside glass wall of the vial to minimize agitation and foaming.
3
Gently rotate or roll the vial until the lyophilized cake is fully dissolved, avoiding shaking.
4
Record the preparation date and resulting concentration (200 mg/mL) on the label, and store between 2–8°C away from light.

Supplies Needed

Peptide Vial

Lyophilized Glutathione 1500 mg container intended for experimental reconstitution

Insulin Syringes (U-100)

Standard 1 mL syringes calibrated at 100 units/mL for accurate volume measurement

Bacteriostatic Water

Sterile diluent preserved with 0.9% benzyl alcohol, utilizing 7.5 mL per vial

Alcohol Swabs

70% isopropyl alcohol wipes for maintaining aseptic conditions on stoppers and surfaces

Why researchers study it

Skin pigmentation and melanogenesis pathways

Endogenous cellular antioxidant defenses and ROS scavenging

Hepatic detoxification and Phase II conjugation mechanisms

Lymphocyte function and intracellular redox regulation

These describe what is being studied, not proven benefits, approved uses, or promised results.

Overview

Glutathione is a low-molecular-weight tripeptide composed of glutamate, cysteine, and glycine, serving as a primary endogenous antioxidant within mammalian biological systems. In laboratory settings, investigators examine its capacity to scavenge reactive oxygen species, regenerate secondary antioxidants like vitamins C and E, and maintain cellular redox equilibrium. Preclinical research frequently focuses on glutathione's vital role as a cofactor for antioxidant enzymes, particularly glutathione peroxidase, as well as its involvement in Phase II hepatic detoxification reactions where it conjugates electrophilic compounds. Experimental models explore how maintaining intracellular pools protects critical cellular architecture and mitochondrial efficiency against oxidative damage. While explored in diverse experimental applications ranging from cellular resilience to skin pigmentation modulation, non-clinical research protocols emphasize careful handling due to molecular susceptibility to oxidation. Synthetic or lyophilized peptide formulations require strict environment controls to maintain thiol group integrity during analytical investigations.

References

1.PubChem / NCBI, Compound Summary, 2024 — Structure and biological roles of the tripeptide glutathione
2.Biochimica et Biophysica Acta, PubMed, 2013 — Glutathione synthesis and maintenance of cellular redox homeostasis
3.U.S. Food & Drug Administration, Guidance, 2019 — Regulatory considerations and alerts on compounded injectable glutathione
4.Antioxidants (Basel), PubMed, 2020 — Cellular defense mechanisms mediated by intracellular thiol antioxidants
5.Free Radical Biology & Medicine, PubMed, 2018 — Enzymatic role of glutathione in cellular detoxification pathways
6.European Journal of Nutrition, PubMed, 2015 — Pharmacokinetics and tissue bioavailability of exogenous glutathione