TB-500 10mg
Synthetic fragment of Thymosin Beta-4 evaluated in preclinical models for actin regulation, wound healing, and soft-tissue repair.
Mechanism
Binds to and sequesters globular actin (G-actin) to regulate cytoskeletal assembly, facilitating directed cell migration, angiogenesis, and extracellular matrix remodeling during tissue repair.
Dosing
Preclinical protocols document an initial daily baseline of 500 mcg administered via subcutaneous injection.
Reconstitution
Introduce 3.0 mL of bacteriostatic water to achieve a final working concentration of approximately 3.33 mg/mL (3,333 mcg/mL).
Storage
Store dry lyophilized vials frozen at -20 °C; once reconstituted, refrigerate at 2–8 °C away from light and do not freeze.
Mix & measure TB-500 10mg
Pre-filled with this protocol's recommended BAC water and documented starting dose — edit any field to run your own numbers.
Mix & measure TB-500 10mg
Vial strength → BAC water → target dose
Total doses in vial: 20.0
U-100 syringe: 100 units = 1 mL
Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Advanced calculator →
Dosing Chart
| Phase / Day(s) | Dose & Frequency | Volume (U-100 units / mL) |
|---|---|---|
| Weeks 1–2 | 500 mcg (1× daily) | ~15 units (0.15 mL) |
| Weeks 3–4 | 600 mcg (1× daily) | ~18 units (0.18 mL) |
| Weeks 5–8 | 750 mcg (1× daily) | ~22.5 units (0.225 mL) |
| Weeks 9–12 | 1000 mcg (1× daily) | ~30 units (0.30 mL) |
Reconstitution Steps
- 1
Draw 3.0 mL of bacteriostatic water into a sterile syringe.
- 2
Release the diluent slowly down the vial's interior glass wall to prevent agitation and foaming.
- 3
Gently roll or swirl the vial until the lyophilized cake is fully dissolved without shaking.
- 4
Label the container with the reconstitution date and concentration, then refrigerate at 2–8 °C protected from light.
Supplies Needed
Peptide Vial
Provides the lyophilized 10 mg TB-500 research material.
Insulin Syringes (U-100)
Facilitates precise measurement and microgram dose extraction.
Bacteriostatic Water
Acts as the sterile multi-dose reconstitution solvent.
Alcohol Swabs
Ensures aseptic preparation of vial septa and injection surfaces.
Why researchers study it
Injury & tissue repair
Wound healing
Anti-inflammatory activity
Angiogenesis & cell migration
These describe what is being studied, not proven benefits, approved uses, or promised results.
Overview
TB-500 is a synthetic peptide mimicking the biological active domain (Ac-LKKTETQ) of Thymosin Beta-4, an endogenous protein critical for cytoskeletal remodeling and systemic cellular migration. In biochemical assays, it demonstrates high-affinity binding to globular actin, modulating monomer sequestration to support cellular motility. Preclinical research frequently explores this fragment for its capacity to accelerate tissue repair, stimulate dermal and corneal wound resolution, and encourage angiogenesis via vascular endothelial growth factor signaling pathways. Unlike the full-length parent protein, which has been evaluated in specific clinical formats, the TB-500 short fragment remains an investigational compound with animal and cellular models forming the bulk of available data. Protocols involving this peptide typically evaluate gradual daily titration schedules to examine physiological responses over an extended experimental period. It is strictly designated for laboratory in vitro and in vivo research and is not intended for human consumption or therapeutic administration.
References
- 1.FASEB Journal, 2012 — Biological activities of thymosin beta-4 mapped to active peptide sequences, including the fragment marketed as TB-500
- 2.Journal of Chromatography A, 2012 — Doping-control analysis of TB-500 as a synthetic thymosin beta-4 fragment in biological samples
- 3.Journal of Investigative Dermatology, 1999 — Thymosin beta-4 accelerates wound healing in a preclinical model
- 4.FASEB Journal, 2007 — Active-site mapping of thymosin beta-4 fragments for angiogenesis and cell migration
- 5.Journal of Investigative Dermatology, 2003 — Thymosin beta-4 wound-healing mechanisms: collagen deposition, angiogenesis and granulation tissue
- 6.Journal of Chromatography B, 2013 — Quantification of TB-500 metabolites and screening of wound-healing activity (prodrug hypothesis)
Supplies Needed
Suggested supplier
Research use only. Listing a supplier is not an endorsement of any protocol on this site, and nothing sold there is approved for human use.
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