Kisspeptin 10mg
Hypothalamic neuropeptide investigated for stimulating endogenous GnRH pulsatility and triggering downstream LH and FSH release.
Mechanism
Kisspeptin activates GPR54 (KISS1R) receptors located on hypothalamic GnRH neurons, driving pulsatile GnRH discharge that subsequently prompts anterior pituitary secretion of LH and FSH.
Dosing
Standard laboratory protocols document an initial dose of 100 mcg administered subcutaneously once daily during the initial titration phase.
Reconstitution
Reconstitution of a 10 mg vial with 3.0 mL of bacteriostatic water yields an approximate working concentration of 3.33 mg/mL (33.3 mcg per U-100 unit).
Storage
Store the unmixed lyophilized peptide frozen at -20 °C, and once reconstituted, maintain refrigerated at 2–8 °C protected from light without refreezing.
Mix & measure Kisspeptin 10mg
Pre-filled with this protocol's recommended BAC water and documented starting dose — edit any field to run your own numbers.
Mix & measure Kisspeptin 10mg
Vial strength → BAC water → target dose
Total doses in vial: 100.0
U-100 syringe: 100 units = 1 mL
Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Advanced calculator →
Dosing Chart
| Phase / Day(s) | Dose & Frequency | Volume (U-100 units / mL) |
|---|---|---|
| Weeks 1–2 | 100 mcg (1× daily) | 3 units (0.03 mL) |
| Weeks 3–4 | 150 mcg (1× daily) | 4.5 units (0.045 mL) |
| Weeks 5–8 | 200 mcg (1× daily) | 6 units (0.06 mL) |
| Weeks 9–12 | 200 mcg (1× daily) | 6 units (0.06 mL) |
Reconstitution Steps
- 1
Draw 3.0 mL of bacteriostatic water into a sterile syringe.
- 2
Introduce the diluent slowly down the interior glass wall of the 10 mg Kisspeptin vial to avoid agitation.
- 3
Gently swirl or roll the vial until the lyophilized cake is fully dissolved, avoiding shaking.
- 4
Mark the vial with reconstitution date and concentration, storing immediately at 2–8 °C away from light.
Supplies Needed
Peptide Vial
Kisspeptin 10 mg lyophilized powder vial for reconstitution (1 to 3 vials depending on protocol duration)
Insulin Syringes (U-100)
Low-volume U-100 syringes (0.3 mL or 0.5 mL preferred) for precise measurement of daily micro-doses
Bacteriostatic Water
Sterile diluent required for mixing (approximately 3.0 mL per 10 mg vial)
Alcohol Swabs
Antiseptic wipes used to sanitize the vial rubber septum and the planned injection site
Why researchers study it
Reproductive hormone cascade stimulation
Fertility and egg-maturation trigger dynamics
Libido and neural emotional processing
Testosterone and luteinizing hormone secretion
These describe what is being studied, not proven benefits, approved uses, or promised results.
Overview
Kisspeptin is an endogenous regulatory neuropeptide derived from the KISS1 gene that plays a central role in governing the hypothalamic-pituitary-gonadal (HPG) axis. By serving as an upstream driver of gonadotropin-releasing hormone (GnRH) release, kisspeptin acts as a primary neuroendocrine switch initiating puberty, sustaining reproductive fertility, and coordinating downstream sexual hormone secretion. In preclinical and clinical research models, exogenous kisspeptin is widely evaluated for its ability to trigger physiological luteinizing hormone (LH) and follicle-stimulating hormone (FSH) pulses without completely overwhelming or bypassing natural hypothalamic feedback loops. This distinct mechanism makes it a key compound of interest in reproductive endocrinology, particularly in studies investigating ovulatory trigger alternatives, hypothalamic amenorrhea, and hypogonadotropic hypogonadism. Beyond reproductive signaling, kisspeptin is subject to exploration regarding its modulation of limbic brain regions involved in libido, mood, and emotional processing. Because it elicits a more natural, self-limiting release of gonadotropins relative to direct GnRH agonists or chorionic gonadotropins, kisspeptin remains a critical research tool for probing neuroendocrine signaling pathways.
References
- 1.Endocrine Reviews (Oxford Academic), 2024 — Comprehensive review of kisspeptin and neurokinin B: role in reproduction and emerging therapeutic potential
- 2.Journal of Clinical Investigation, 2014 — Kisspeptin-54 triggers oocyte maturation in IVF with reduced ovarian-hyperstimulation risk
- 3.ClinicalTrials.gov, 2024 — Clinical-trial registry for kisspeptin reproductive research and investigational protocols
Supplies Needed
Suggested supplier
Research use only. Listing a supplier is not an endorsement of any protocol on this site, and nothing sold there is approved for human use.
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