HCG 5000 IU
Human chorionic gonadotropin acts as an LH mimetic to support testicular Leydig cell function, spermatogenesis, and hormonal regulation.
Mechanism
HCG functions as an agonist at the LH/CG receptor, mimicking luteinizing hormone to stimulate testicular Leydig cell testosterone production and gonadal endocrine pathways.
Dosing
Standard maintenance protocols document a starting dose of 500 IU administered subcutaneously three times per week.
Reconstitution
Reconstituting a 5000 IU vial with 2.0 mL of bacteriostatic water yields a final concentration of 2,500 IU per milliliter.
Storage
Store unmixed lyophilized powder refrigerated between 2 to 8 degrees Celsius, and maintain the reconstituted liquid at the same temperature for up to sixty days without freezing.
Mix & measure HCG 5000 IU
Pre-filled with this protocol's recommended BAC water and documented starting dose — edit any field to run your own numbers.
Mix & measure HCG 5000 IU
Vial strength → BAC water → target dose
Total doses in vial: 10.0
U-100 syringe: 100 units = 1 mL
Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Advanced calculator →
Dosing Chart
| Phase / Day(s) | Dose & Frequency | Volume (U-100 units / mL) |
|---|---|---|
| Standard Maintenance (Weeks 1–12) | 500 IU (3× weekly, Mon/Wed/Fri) | 20 units (0.20 mL) |
| High-Dose Phase 1 (Weeks 1–4) | 1,500 IU (3× weekly) | 60 units (0.60 mL) |
| High-Dose Phase 2 (Weeks 5–8) | 2,000 IU (3× weekly) | 80 units (0.80 mL) |
| High-Dose Phase 3 (Weeks 9–12) | 1,000 IU (3× weekly) | 40 units (0.40 mL) |
Reconstitution Steps
- 1
Aspirate 2.0 mL of sterile bacteriostatic water using a fresh syringe.
- 2
Introduce the diluent slowly down the glass wall of the HCG vial to minimize agitation and foaming.
- 3
Gently rotate or roll the vial between your fingers until the lyophilized cake is fully dissolved.
- 4
Record the reconstitution date and the 2,500 IU/mL concentration on the vial label.
- 5
Place the reconstituted solution into refrigeration at 2–8 °C immediately.
Supplies Needed
Peptide Vial
HCG 5000 IU lyophilized powder vial for reconstitution.
Insulin Syringes (U-100)
Calibrated 1 mL U-100 syringes used to accurately draw and administer 20-unit (0.20 mL) doses.
Bacteriostatic Water
Sterile diluent (2.0 mL per vial) containing benzyl alcohol to dissolve the peptide and inhibit microbial growth.
Alcohol Swabs
Antiseptic wipes used to sterilize the rubber vial stopper and the subcutaneous preparation site.
Why researchers study it
Testosterone support and Leydig cell activation
Fertility preservation and spermatogenesis maintenance
Preservation of testicular volume during suppression
Ovulation induction and luteal function modeling
These describe what is being studied, not proven benefits, approved uses, or promised results.
Overview
Human chorionic gonadotropin (HCG) is a complex heterodimeric glycoprotein hormone that shares structural and functional homology with luteinizing hormone (LH). By selectively targeting and binding the shared LH/CG receptor, it plays a vital role in stimulating steroidogenesis within gonadal tissue. Unlike endogenous pituitary LH, HCG features an extended circulating half-life of roughly thirty-six hours, making it an effective candidate for maintaining steady signaling pathways. In preclinical and clinical research models, HCG is widely evaluated for its capacity to sustain intratesticular testosterone levels and preserve spermatogenesis, especially during states of pituitary-gonadal axis suppression. Furthermore, it is explored in female reproductive research settings as a potent signaling molecule to mimic the mid-cycle LH surge necessary for oocyte maturation and ovulation.
References
- 1.National Center for Biotechnology Information (NCBI), StatPearls, 2023 — Human chorionic gonadotropin: structure, function and LH/CG receptor binding mechanism
- 2.Seminars in Reproductive Medicine, Sem Reprod Med, 2014 — Pharmacokinetics and pharmacodynamics of HCG: extended half-life and gonadal activity
- 3.Lee JA & Ramasamy R, Translational Andrology and Urology, 2018 — hCG for hypogonadal male infertility regimens
- 4.Coviello AD et al., J. Clinical Endocrinology & Metabolism, 2005 — Low-dose hCG maintains intratesticular testosterone during gonadotropin suppression
- 5.FDA Prescribing Information, FDA Drug Label, 2009 — Chorionic Gonadotropin (Pregnyl/Novarel) stability, storage, and indications
- 6.Centers for Disease Control and Prevention (CDC), Vaccine Administration Protocols, 2023 — Subcutaneous administration and injection technique standards
Supplies Needed
Suggested supplier
Research use only. Listing a supplier is not an endorsement of any protocol on this site, and nothing sold there is approved for human use.