GHK (Copper-Free) 50mg

1. 1

   Clean the rubber stoppers on both the GHK vial and the bacteriostatic water container using sterile alcohol swabs and allow them to air-dry completely.

2. 2

   Aspirate 2.0 mL of bacteriostatic water using a sterile mixing syringe.

3. 3

   Insert the needle at a slight angle and discharge the diluent gently against the inner glass wall of the peptide vial.

4. 4

   Allow the mixture to rest for 30 seconds, then roll the vial smoothly between your hands until the lyophilizate fully dissolves without shaking.

5. 5

   Inspect the clear or faintly tinted blue-purple solution for particulate matter and place in refrigeration at 2–8°C.

Overview

GHK (Copper-Free), commonly designated as GHK Basic, is the unchelated form of the naturally occurring human tripeptide glycyl-L-histidyl-L-lysine. Originally isolated from human plasma albumin fractions, the tripeptide is traditionally studied for its roles in tissue regeneration, dermal remodeling, and cellular signaling. While commercially prepared without an incorporated metal ion, GHK possesses a profound affinity for divalent copper ions comparable to endogenous transport proteins, meaning it rapidly complexes with ambient trace copper in biological matrices to form GHK-Cu. In laboratory literature, uncomplexed GHK is utilized to examine fundamental peptide-metal interactions, baseline cellular proliferation, and comparative transport mechanics without pre-existing copper saturation. Because clinical trials have historically examined topical formulations of the copper complex rather than injectable native peptides, research into copper-free GHK primarily assesses how the naked peptide recruits metal cofactors and affects fibroblast signaling in controlled experimental setups.

1. 1.Pickart, Journal of Biomaterials Science, Polymer Edition, 2008 — The human tri-peptide GHK and tissue remodeling
2. 2.Pickart et al., Oxidative Medicine and Cellular Longevity, 2012 — The human tripeptide GHK-Cu in prevention of oxidative stress and degenerative conditions of aging
3. 3.Choi et al., Journal of Peptide Science, 2012 — Stem cell recovering effect of copper-free GHK in skin
4. 4.Maquart et al., FEBS Letters, 1988 — Stimulation of collagen synthesis in fibroblast cultures by the tripeptide-copper complex GHK-Cu2+
5. 5.Maquart et al., Journal of Clinical Investigation, 1993 — In vivo stimulation of connective tissue accumulation by GHK-Cu2+ in rat experimental wounds
6. 6.Wegrowski et al., Life Sciences, 1992 — Stimulation of sulfated glycosaminoglycan synthesis by the tripeptide-copper complex GHK-Cu2+
7. 7.Fu et al., Journal of Orthopaedic Research, 2015 — GHK-Cu(II) transiently improved healing in a rat model of ACL reconstruction
8. 8.Ma et al., Life Sciences, 2019 — Protective effects of GHK-Cu in bleomycin-induced pulmonary fibrosis
9. 9.Badenhorst et al., Pharmaceutical Development and Technology, 2016 — Physicochemical characterization of native GHK: a preformulation study for dermal delivery
10. 10.Ogórek et al., Molecules, 2025 — Skin permeation dynamics of GHK-Cu tripeptide formulations
11. 11.Pickart & Margolina, International Journal of Molecular Sciences, 2018 — Regenerative and Protective Actions of the GHK-Cu Peptide in the Light of the New Gene Data

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