SingleLongevityNew

Epithalon 10mg

Synthetic pineal tetrapeptide studied for telomerase upregulation, circadian restoration, and cellular longevity.

Mechanism

Epithalon stimulates telomerase enzymatic activity via hTERT transcription and modulates pineal neuroendocrine pathways to support cellular longevity and circadian homeostasis.

Dosing

Standard laboratory longevity protocols examine 5 mg daily over brief intervention courses.

Reconstitution

Add 2.0 mL bacteriostatic water to one 10 mg vial to produce a final working concentration of 5 mg/mL.

Storage

Store lyophilized powder sealed at −20 °C; once reconstituted, refrigerate at 2–8 °C and protect from light.

Mix & measure Epithalon 10mg

Pre-filled with this protocol's recommended BAC water and documented starting dose — edit any field to run your own numbers.

Mix & measure Epithalon 10mg

Vial strength → BAC water → target dose

Vial strength

BAC water

Target dose

Concentration5.00mg/mL

Draw volume1.00mL

On the syringe100.0units

Total doses in vial: 2.0

100u80u60u40u20u0

U-100 syringe: 100 units = 1 mL

Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Advanced calculator →

Dosing Chart

Phase / Day(s)	Dose & Frequency	Volume (U-100 units / mL)
Days 1–10 (Standard Run)	5 mg once daily	100 units (1.0 mL)
Days 11–20 (Extended Course)	5 mg once daily	100 units (1.0 mL)
Maintenance Interval	0 mg (Off-cycle research period)	0 units (0.0 mL)

Reconstitution Steps

1
Remove the 10 mg Epithalon vial from the -20 °C storage freezer.
2
Allow the vial to equilibrate sealed at room temperature for approximately 15–20 minutes.
3
Draw exactly 2.0 mL of bacteriostatic water into a sterile syringe.
4
Inject the diluent slowly down the interior glass vial wall to prevent aerosolization or agitation.
5
Swirl and roll the vial gently between hands until the lyophilized cake is completely dissolved and clear; do not invert or shake.
6
Label the vial with date, preparation details, and resulting concentration (5 mg/mL), then store in the refrigerator at 2–8 °C.

Supplies Needed

Peptide Vial

Contains 10 mg of lyophilized Epithalon cake; one vial yields two 5 mg research doses.

Insulin Syringes (U-100)

Used for precise measurement and subcutaneous administration of 1.0 mL (100 units) per dose.

Bacteriostatic Water

Sterile diluent containing 0.9% benzyl alcohol; 2.0 mL is required per 10 mg vial.

Alcohol Swabs

Used for sanitizing rubber vial stoppers and research injection sites prior to every draw.

Why researchers study it

Telomerase activation and hTERT induction

Pineal gland bioregulation and melatonin dynamics

Cellular senescence and lifespan extension models

Circadian rhythm and sleep architecture restoration

These describe what is being studied, not proven benefits, approved uses, or promised results.

Overview

Epithalon (also known as Epitalon or Ala-Glu-Asp-Gly) is a synthetic tetrapeptide modeled after epithalamin, a bioregulatory peptide complex initially isolated from bovine pineal gland tissue. Originally developed at the St. Petersburg Institute of Bioregulation and Gerontology, the synthetic sequence was identified as the key functional component responsible for pineal-driven geroprotective actions observed in preliminary animal and cellular models. From a mechanistic perspective, Epithalon has attracted significant scientific interest due to its capacity to induce human telomerase reverse transcriptase (hTERT) gene expression and activate telomerase in somatic cells, counteracting progressive telomeric attrition. Beyond telomerase induction, investigation has revealed regulatory roles in pineal neuroendocrine pathways, including normalization of endogenous melatonin synthesis, stabilization of circadian rhythmicity, and induction of endogenous antioxidant defense enzymes such as superoxide dismutase. Currently, Epithalon remains an investigational research peptide utilized strictly within preclinical and clinical longevity research contexts. Although substantial cell-culture findings and animal longevity assays demonstrate delayed cellular senescence, modern Western regulatory validation and broad multi-center randomized controlled trials remain areas for continued inquiry.

References

1.Khavinson VK, Bondarev IE, Butyugov AA, Bulletin of Experimental Biology and Medicine, 2003 — Epithalon peptide induces telomerase activity and telomere elongation in human somatic cells.
2.Anisimov VN, Khavinson VK, Biogerontology, 2010 — Peptide bioregulation of aging: results and prospects.
3.Araj SK et al., International Journal of Molecular Sciences, 2025 — Overview of Epitalon: highly bioactive pineal tetrapeptide and its geroprotective roles.
4.Khavinson VK et al., Mechanisms of Ageing and Development, 2024 — Epitalon increases telomere length in human cell lines through telomerase upregulation or ALT activity.