SingleLongevity

NAD+ 500mg

Essential metabolic coenzyme studied for sustaining cellular bioenergetics, mitochondrial respiration, and longevity pathways.

Mechanism

Acts as an essential metabolic electron transporter in mitochondrial ATP synthesis and an obligatory cosubstrate for PARP and sirtuin enzymes governing DNA repair and cellular longevity.

Dosing

Standard laboratory reference regimens initiate exploration at 50 mg administered subcutaneously once daily, titrating gradually up to 100 mg daily as tolerated.

Reconstitution

Reconstituting a 500 mg vial with 5.0 mL of bacteriostatic water yields an exact working concentration of 100 mg/mL, simplifying syringe volume calibration to 1 mg per U-100 unit.

Storage

Store the unmixed lyophilized powder at -20 °C protected from moisture; upon reconstitution, maintain refrigerated at 2 to 8 °C in the dark and avoid repeated freeze-thaw cycles.

Mix & measure NAD+ 500mg

Pre-filled with this protocol's recommended BAC water and documented starting dose — edit any field to run your own numbers.

Mix & measure NAD+ 500mg

Vial strength → BAC water → target dose

Vial strength

BAC water

Target dose

Concentration100.0mg/mL

Draw volume0.500mL

On the syringe50.0units

Total doses in vial: 10.0

100u80u60u40u20u0

U-100 syringe: 100 units = 1 mL

Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Advanced calculator →

Dosing Chart

Phase / Day(s)	Dose & Frequency	Volume (U-100 units / mL)
Weeks 1–2	50 mg (1× daily)	50 units (0.50 mL)
Weeks 3–4	75 mg (1× daily)	75 units (0.75 mL)
Weeks 5–8	100 mg (1× daily)	100 units (1.0 mL)
Weeks 9–12	100 mg (1× daily)	100 units (1.0 mL)

Reconstitution Steps

1
Aseptically draw 5.0 mL of bacteriostatic water into a sterile syringe.
2
Introduce the diluent slowly against the inside glass wall of the NAD+ vial to minimize turbulence and foaming.
3
Swirl or rotate the vial gently until the cake is fully dissolved, strictly avoiding vigorous shaking.
4
Label the reconstituted vial with the date and resulting concentration (100 mg/mL).
5
Refrigerate the solution at 2–8 °C, shielded from ambient light sources.

Supplies Needed

Peptide Vial

NAD+ 500 mg lyophilized research vial

Insulin Syringes (U-100)

1 mL (U-100) sterile syringes for accurate measurement and administration

Bacteriostatic Water

5.0 mL diluent per 500 mg vial for standard reconstitution

Alcohol Swabs

Antiseptic prep pads for vial stoppers and administration sites

Why researchers study it

Longevity and mitigation of cellular aging

Cellular energy generation and metabolic pathways

Mitochondrial respiratory function and redox balance

Cognitive preservation and neuroprotective models

These describe what is being studied, not proven benefits, approved uses, or promised results.

Overview

Nicotinamide adenine dinucleotide (NAD+) is a vital endogenous coenzyme present across all living cells rather than an authentic peptide. It operates at the heart of cellular energy metabolism, functioning as a primary electron carrier in central oxidative pathways including glycolysis, the tricarboxylic acid cycle, and mitochondrial oxidative phosphorylation. Beyond basic cellular respiration, NAD+ serves as an obligatory cosubstrate for critical regulatory enzymes such as sirtuins (SIRTs) and poly(ADP-ribose) polymerases (PARPs). Through these pathways, cellular NAD+ availability heavily influences DNA repair mechanisms, genomic stability, inflammatory signaling, and chromatin regulation. Preclinical observations consistently indicate that endogenous NAD+ pools decline throughout the natural aging process and under states of persistent metabolic stress. Consequently, exogenous administration protocols are widely investigated in experimental models to evaluate potential restorative effects on mitochondrial resilience, systemic metabolic health, and neuroprotection.

References

1.Pharmaceuticals (Basel), 2020 — Clinical evidence for targeting NAD+ therapeutically: metabolic pathways and therapeutic potential
2.PMC8612620, 2021 — Comprehensive review of NAD+ metabolism, mitochondrial function and aging
3.Grant et al., Frontiers in Aging Neuroscience, 2019 — Pilot study of human plasma and urine NAD+ metabolome during a 6-hour IV infusion (750 mg)
4.Braidy et al., PMC8612620, 2021 — Pharmacology and potential implications of NAD+ metabolism, aging and therapeutics